Preclinical Research Projects

GABAergic Mechanisms in Schizophrenia

Miwa H, et al.
Glutamate decarboxylase 67 deficiency in a subset of GABAergic veurons induces schizophrenia-related phenotypes.
Neuropsychopharmacol 40, 2475-2486, 2015.
(Published from Gunma University)

Decreased expression of the GABA synthetic enzyme glutamate decarboxylase 67 (GAD67) in a subset of GABAergic neurons, including parvalbumin (PV)-expressing neurons, has been observed in postmortem brain studies of schizophrenics and in animal models of schizophrenia. However, it is unclear whether and how the perturbations of GAD67-mediated GABA synthesis and signaling contribute to the pathogenesis of schizophrenia. To address this issue, we generated the mice lacking GAD67 primarily in PV neurons and characterized them with focus on schizophrenia-related parameters.


Neurodevelopmental hypothesis of schizophrenia

Furuie H, et al.
Differential effects of N-methyl-D-aspartate receptor blockade during the second and third postnatal weeks on spatial working and reference memory in adult rats.
Brain Res 1721, 2019.

Early postnatal glutamatergic NMDA receptor blockade in animals is known to produce various behavioral deficits in adulthood. In the present study rats postnatally (day 7-20) treated chronically with MK-801, an NMDA receptor antagonist, were tested later in adulthood in methamphetamine (MAP)-induced conditioned place preference (CPP) using a unbiased procedure in a three-compartment apparatus. Rats with the same chronic treatment in adulthood were also tested. Our results suggest the possibility that chronic early postnatal, but not adulthood, NMDA receptor blockade induces persistent deficit of subsequent appetitive classical conditioning.


The roles of the orbitofrontal Cortex in the pathophisiology of psychiatric disorders.
Optogenetic and chemogenetic approaches.

Kuniishi H, et al.
Stress induces insertion of calcium-permeable AMPA receptors in the OFC–BLA synapse and modulates emotional behaviours in mice.
Translational Psychiatry 10: 154, 2020.

The orbitofrontal cortex (OFC) is involved in emotional processing, and orbitofrontal abnormalities have often been observed in various affective disorders. Thus, chronic dysfunction of the OFC may cause symptoms of affective disorders, such as anxiety, depression and impulsivity. Previous studies have investigated the effect of orbitofrontal dysfunction on anxiety-like behavior and impulsive aggression in rodents, but the results are inconsistent possibly reflecting different methods of OFC inactivation. Therefore, we are currently investigating the roles of the OFC in the pathophisiology of psychiatric disorders using optogenetic and chemogenetic approaches.


Chronic social defeat stress model focusing on the psychological stress:
The social defeat witness model

Nakatake Y, et al.
The effects of emotional stress are not identical to those of physical stress in mouse model of social defeat stress.
Neurosci Res in press, 2020. (Available online 16 October 2019)

Chronic social defeat stress (CSDS) models in rodents are often used for researches on stress-related psychiatric disorders. CSDS includes emotional and physical aspects of stress and it is difficult to distinguish these two components. However, in many cases, patients are affected solely by emotional rather than physical distress. Recently, we have established a new stress model focusing on the emotional stress using witnessing the defeat of a conspecific. In this study, we examined the effect of emotional stress on the behavior and the plasma cytokine levels in mice.


Pharmacplogical intervention to regulate the extinction and reconsolidation of fear memory

Akagi K, et al.
Post-reexposure administration of riluzole attenuates the reconsolidation of conditioned fear memory in rats.
Neuropharmacol 131, 1-10, 2018.

Recently, we demonstrated that riluzole, which has been shown to block the glutamatergic system, facilitates fear extinction in rats. Here, we undertook experiments on contextual fear conditioning to clarify the actions of riluzole on the reconsolidation of fear memory in rats. We demonstrated that riluzole impaired the reconsolidation of contextual fear memory. Results from spontaneous recovery experiments also suggested that riluzole attenuated reconsolidation. Indeed, conditioned fear did not recover spontaneously 4 weeks after a short reexposure and riluzole administration, whereas it recovered after a long reexposure. Using western blotting, we demonstrated that a short reexposure increased the phosphorylation of cyclic adenosine monophosphate response element binding protein significantly in the dorsal part of hippocampus. Interestingly, this phosphorylation was attenuated by riluzole with short reexposure. In addition, bilateral microinjection of riluzole directly into the dorsal hippocampus clearly attenuated the reconsolidation. These findings suggested that the attenuating effect of riluzole on the reconsolidation of fear memory involves, at least in part, the dorsal part of the hippocampus.