Koike S, Shibuya N, Kimura H, Ishii K, Ogasawara Y. Polysulfide promotes neuroblastoma cell differentiation by accelerating calcium influx. Biochem. Biophys. Res. Commun.459, 488-492, 2015.

Kimura, Y., Toyofuku, Y., Koike, S., Shibuya, N., Nagahara, N., Lefer, D., Ogasawara, Y., Kimura, H. Identification of H2S3 and H2S produced by 3-mercaptopyruvate sulfurtransferase in the brain. Sci. Rep. 5: 24774, 2015.

Hatakeyama, Y., Takahashi, K., Tominaga, M., Kimura, H., Ohta, T. Polysulfide evokes acute pain through the activation of nociceptive TRPA1 in mouse sensory neurons. Mol. Pain 11, 24, 2015

Raveney B., Oki S., Hohjoh H., Nakamura M., Sato W., Murata M., and Yamamura T. (2015) Eomesodermin-expressing T helper cells are essential for chronic neuroinflammation. Nat Commun, 6: 8437.

Adachi N., Numakawa T., Nakajima S., Fukuoka M., Odaka H., Katanuma Y., Ooshima Y., Hohjoh H., and Kunugi H. (2015) Glucocorticoid affects dendritic transport of BDNF-containing vesicles. Sci Rep, 5: 12684.

Takahashi M., Suzuki M., Fukuoka M., Fujikake N., Watanabe S., Murata M., Wada K., Nagai Y., and Hohjoh H. (2015) Normalization of overexpressed alpha-synuclein causing Parkinson’s disease by a moderate gene silencing with RNA interference. Mol Ther Nucleic Acids, 4: e241.

Kimura, H. Signaling of hydrogen sulfide and polysulfides. (Forum Editorial) Antioxid. Redox Signal. 22, 347-349, 2015.

Kimura, H. Signaling molecules: hydrogen sulfide and polysulfide. Antioxid. Redox Signal. 22, 362-376, 2015.

Kimura, H. H2S2014 in Kyoto the 3rd international conference on H2S in biology and medicine. Nitric Oxide 46, 1-6, 2015.

Kimura H. Hydrogen fulfide and polysulfides as signaling molecules. Proc. Jpn. Acad., Ser. B 91, 131-159, 2015.

木村英雄 硫化水素とポリサルファイドの生理機能。日薬理誌 147、23-29、2016.

北條浩彦:ずくなしのミニプレップ.実験医学 33: 3161-3165, 2015.

北條浩彦:ずくなしのトランスフォーメーション法.実験医学 33: 1456-1460, 2015.


Takahashi M. and Hohjoh H. (2014) A novel measurement of allele discrimination for assessment of allele-specific silencing by RNA interference. Mol Biol Rep, 41: 7115-7120.

Fukuoka M., Yoshida M., Eda A., Takahashi M., Hohjoh H. (2014) Gene silencing mediated by endogenous microRNAs under heat stress conditions in mammalian cells. PLoS ONE, 9: e103130.

Araki W., Minegishi S., Motoki K., Kume H., Hohjoh H., Araki YM., and Tamaoka A. (2014) Disease-associated mutations of TDP-43 promote turnover of the protein through the proteasomal pathway. Mol Neurobiol, 50: 1049-1058.

Kimura, H. Production and physiological effects of hydrogen sulfide. Antioxid. Redox Signal. 20, 783-793, 2014.

Kimura, H. Hydrogen sulfide and polysulfides as biological mediators. Molecules 19, 16146-16157, 2014.

Kimura, H. The physiological role of hydrogen sulfide and beyond. Nitric Oxide: Biol. Chem., 41, 4-10, 2014.

木村英雄 硫化水素(H2S)とポリサルファイド(H2Sn)の生理機能。アミノ酸研究 8, 199-213, 2014.


Shibuya N, Koike S, Tanaka M, Ishigami-Yuasa M, Kimura Y, Ogasawara Y, Fukui K, Nagahara N, Kimura H.  A novel pathway for the production of hydrogen sulfide from D-cysteine in mammalian cells. Nat. Commun. 4, 1366, 2013. 読売新聞等に掲載


Kimura Y, Mikami Y, Osumi K, Tsugane M, Oka J-I, Kimura H.  Polysulfides are possible H2S-derived signaling molecules in rat brain.  FASEB J. 27, 2451-2457, 2013. 日本経済産業新聞に掲載


Koike S, Ogasawara Y, Shibuya N, Kimura H, Ishii K.  Polysulfide exerts a protective effect against cytotoxicity caused by t-buthylhydroperoxide through Nrf2 signaling in neuroblastoma cells. FEBS Lett. 587, 3548-3555, 2013.


Mikami Y, Shibuya N, Ogasawara Y, Kimura H.  Hydrogen sulfide is produced by cystathionineγ–lyase at the steady-state low intracellular Ca2+ concentrations. Biochem. Biophys. Res. Commun. 431, 131-135, 2013.


Ogasawara Y, Hirokawa T, Matsushima K, Koike S, Shibuya N, Tanabe S, Ishii K: A novel method for the analysis of 3-mercaptopyruvate using high-performance liquid chromatography with fluorescence detection. J Chromatogr B Analyt Technol Biomed Life Sci 931, 56-60, 2013


Takahashi M., Chiyo T., Okada T., and Hohjoh H. Specific inhibition of tumor cells by oncogenic EGFR specific silencing by RNA interference. PLoS ONE, 8(8): e73214. 2013.


Hohjoh H. Disease-causing allele-specific silencing by RNA interference. Pharmaceuticals, 6: 522-535. 2013.


Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., and Wada K. Ubiquitin C-terminal hydrolase L1 (UCH-L1) acts as novel potentiator of cyclin-dependent kinases to enhance cell proliferation, independent of its hydrolase activity. J Biol Chem, 288: 12615-12626. 2013.


Shin M., Ohte S., Fukuda T., Sasanuma H., Yoneyama K., Kokabu S., Miyamoto A., Tsukamoto S., Hohjoh H., Jimi E., and Katagiri T. Identification of a novel bone morphogenetic protein (BMP)-inducible transcript, BMP-inducible transcript-1, by utilizing the conserved BMP-responsive elements in the Id genes. J Bone Miner Metab, 31: 34-43. 2013.


Shibuya N, Kimura H. Production of hydrogen sulfide from D-cysteine and its therapeutic potential.  Frontiers in Endocrinology 4, article 87, 2013.


Ingenbleek Y, Kimura H. Nutritional essentiality of sulfur in health and diesease. Nutr. Rev. 71, 413-432, 2013.


Kimura H. Phydiological role of hydrogen sulfide and polysulfide in the central nervous system. Neurochem. Int. 63, 492-497, 2013.


木村英雄:硫化水素(H2S)の生理機能と医療応用 生化学 85(2),63-75,2013


渋谷典広,木村英雄:生理活性物質としての硫化水素 DOJIN News NO.146,1-6,2013


木村由佳,木村英雄:生理活性物質硫化水素H2S)の医療応用 医薬品医療機器レギュラトリーサイエンス,PMDRS,44(3),190-199(2013)



Taniguchi, S., Kimura, T., Umeki, T., Kimura, Y., Kimura, H., Ishii, I., Itoh, N., Naito, Y., Ymamoto, H., Niki, I.  Protein phosphorylation involved in the gene expression of the hydrogen sulphide producing enzyme cystathionine g-lyase in the pancreatic b-cell.  Molecular and Cellular Endochrinology, 350, 31-38, 2012.


Tokuda, K., Kida, K., Marutani, E., Crimi, E., Bougaki, M., Khatri, A., Kimura, H., and Ichinose, F.  Inhaled hydrogen sulfide prevents endotoxin-induced systemic inflammation and improves survival by altering sulfide metabolism in mice.  Antioxid. Redox Signal. 17, 11-21, 2012.


Takahashi M., Eda A., Fukushima T., and Hohjoh H. Reduction of type IV collagen by upregulated miR-29 in normal elderly mouse and klotho-deficient, senescence-model mouse. PLoS ONE, 7(11): e48974, 2012.


Hamasaki M., Hashizume Y., Yamada Y., Katayama T., Hohjoh H., Fusaki N., Nakashima Y., Furuya H., Haga N., Takami Y., and Era T. Pathogenic mutation of ALK2 inhibits iPS cell reprogramming and maintenance: mechanisms of reprogramming and strategy for drug identification. Stem Cells, 30: 2437-2449, 2012.


Takahashi M., Katagiri T., Furuya H., and Hohjoh H. Disease-causing allele specific silencing against the ALK2 mutants, R206H and G356D, in Fibrodysplasia Ossificans Progressiva. Gene Therapy, 19: 781-785, 2012.


Ohnishi Y., Totoki Y., Toyoda A., Watanabe T., Yamamoto Y., Tokunaga K., Sakaki Y., Sasaki H., and Hohjoh H. Active role of small non-coding RNAs derived from SINE/B1 retrotransposon during early mouse development. Mol Biol Rep, 39: 903-909, 2012.


Kimura, H. Metabolic turnover of hydrogen sulfide. Frontiers in Physiology 3, article 101, 1-3 (2012).


Kimura, H., Shibuya, N., Kimura, Y.  Hydrogen sulfide is a signaling molecule and a cytoprotectant.  Antioxid. Red. Signal. 17, 45-57, 2012.


Mikami, Y., and Kimura, H. A mechanism of retinal protection from light-induced degeneration by hydrogen sulfide. Commun. Integr. Biol. 5, 169-171, 2012.


木村英雄:シグナル分子および細胞保護因子としての硫化水素.日本薬理学雑誌 139, 5, 2012


木村英雄:硫化水素:生合成、放出、生理機能.日本薬理学雑誌 139, 6-8, 2012


渋谷典広,木村英雄:硫化水素H2S)の基礎 LiSA 19(12),1284-1289,2012



Mikami Y, Shibuya N, Kimura Y, Nagahara N, Ogasawara Y, Kimura H: Thioredoxin and dihydrolipoic acid are required for 3-mercaptopyruvate sulfurtransferase to produce hydrogen sulfide. Biochem J 439: 479-485, 2011


Mikami Y, Shibuya N, Kimura Y, Nagahara N, Yamada M, Kimura H: Hydrogen sulfide protects the retina from light-induced degeneration by the modulation of Ca2+ influx. J Biol Chem 286: 39379-39386, 2011


Sasakura K, Hanaoka K, Shibuya N, Mikami Y, Kimura Y, Komatsu T, Ueno T, Terai T, Kimura H, Nagano T: Development of a highly selective fluorescence probe for hydrogen sulfide. J Am Chem Soc 133: 18003-18005, 2011


Eda A, Takahashi M, Fukushima T, and Hohjoh H: Alteration of microRNA expression in the process of mouse brain growth. Gene 485: 46-52, 2011


Kimura H: Hydrogen sulfide: its production, release and functions. Amino Acids 41: 113-121, 2011


Rong W, Kimura H, Grundy D: The neurophysiology of hydrogen sulfide. Inflamm Allergy Drug Targets 10: 109-117, 2011


Kimura H: Hydrogen sulfide: its production and functions. Exp Physiol 96: 833-835, 2011


高橋理貴、北條浩彦: トリプレット・リピート病原因遺伝子に対するテーラーメイドRNAiノックダウン.実験医学 29: 931-934, 2011



Kimura, Y., Goto, Y., and Kimura, H.  Hydrogen sulfide increases    glutathione production and suppresses oxidative stress in mitochondria.  Antioxid. Red. Signal. 12, 1-13, 2010.

Namekata, K., Harada, C., Taya, C., Guo, X., Kimura, H., Parada, L.F., and Harada, T.  Dock3 induces axonal outgrowth by stimulating membrane recruitment of the WAVE complex.  Proc. Natl. Acad. Sci. USA. 107, 7586-7591, 2010.

Takahashi M., Watanabe S., Murata M., Furuya H., Kanazawa I., Wada K., and Hohjoh H.: Tailor-made RNAi knockdown against triplet repeat disease-causing alleles. Proc Natl Acad Sci USA., 107: 21731-21736, 2010.


Ohnishi Y., Totoki Y., Toyoda A., Watanabe T., Yamamoto Y., Tokunaga K., Sakaki Y., Sasaki H., and Hohjoh H.: Small RNA class transition from siRNA/piRNA to miRNA during pre-implantation mouse development. Nucl. Acids Res., 38: 5141-5151, 2010.


Hohjoh H.: Allele-specific silencing by RNA interference. In RNA interference. Methods Mol. Biol., 623: 67-79, 2010.



Ishigami, M., Hiraki, K., Umemura, K., Ogasawara, Y., Ishii, K. and Kimura, H. A source of hydrogen sulfide and a mechanism of its release in the brain.  Antioxid. Red. Signal. 11, 205-214 2009.


Shibuya, N., Tanaka, M, Yoshida, M., Ogasawara, Y., Togawa, T., Ishii, K. and Kimura, H. 3-Mercaptopyyruvate sulfurtransferase produces hydrogen sulfide and bound sulfane sulfur in the brain.  Antioxid. Red. Signal.11, 703-714, 2009.


Kaneko, Y., Kimura, T., Taniguchi, S., Souma, M., Kojima Y., Kimura Y., Kimura, H. and Niki, I.  Glucose-induced production of hydrogen sulfide may protect the pancreatic beta-cells from apoptotic cell death by high glucose.  FEBS Lett. 585, 377-382, 2009.


Takei, N., Miyashita, A., Tsukie, T., Arai, H., Asada, T., Imagawa, M., Shoji, M., Higuchi, S., Urakami, K., Kimura, H., Kakita, A., Takahashi, H., Tsuji, S., Kanazawa, I., Ihara, Y., Odani, S., Kuwano, R. and the Japanese Genetic Study Consortium for Alzheimer Disease.  Genetic association study on in and around the APOE in late-onset Alzheimer disease in Japanese. Genomics 93, 441-448, 2009.


Shibuya, N., Mikami, Y., Kimura, Y., Nagahara, N., and Kimura, H.Vascularendotheliumexpresses3-mercaptopyruvate sulfurtransferase and produces hydrogen sulfide.  J. Biochem. 146, 623-626, 2009.


Eda A., Tamura Y., Yoshida M., and Hohjoh H.: Systematic gene regulation involving miRNAs during neuronal differentiation of mouse P19 embryonic carcinoma cell. BBRC, 388: 648-653, 2009.


Kitamura K., Itou Y., Yanazawa M., Ohsawa M., Suzuki-Migishima R., Umeki Y., Hohjoh H., Yanagawa Y., Shinba T., Itoh M., Nakamura K., and Goto Y.: Three human ARX mutations cause the lissencephaly-like and mental retardation with epilepsy-like pleiotropic phenotypes in mice. Hum. Mol. Genet., 18: 3708-3724, 2009.


Hohjoh H., Akari H., Fujiwara Y., Tamura Y., Hirai H., and Wada K.: Molecular cloning and characterization of the common marmoset huntingtin gene. Gene, 432: 60-66, 2009.


Tamura Y., Yoshida M., Ohnishi Y., and Hohjoh H.: Variation of gene silencing involving endogenous microRNA in mammalian cells. Mol Biol Rep, 36: 1413-1420, 2009.



Tamura Y., Yoshida M., Ohnishi Y., and Hohjoh H.: Variation of gene silencing involving endogenous microRNA in mammalian cells. Mol Biol Rep, 2008 Aug 12. [Epub ahead of print].


Doi Y., Oki S., Ozawa T., Hohjoh H., Miyake S., and Yamamura T.: Orphan nuclear receptor NR4A2 expressed in T cells from multiple sclerosis mediates production of inflammatory cytokines. Proc Natl Acad Sci USA., 105: 8381-8386, 2008.


Ohnishi Y., Tamura Y., Yoshida M., Tokunaga K., and Hohjoh H.: Enhancement of allele discrimination by introduction of nucleotide mismatches into siRNA in allele-specific gene silencing by RNAi. PLoS ONE 3(5): e2248, 2008.



Elrod, JW., Calvert, JW., Morrison, J., Doeller, JE, Kraus, DW., Tao, L., Jiao, X., Scalia, R., Kiss, L., Szabo, C., Kimura, H., Chow, CW., Lefer, DJ.  Hydrogen sulfide attenuates myocardial ischemia-reperfusion injury by preservation of mitochondrial function.  Proc. Natl. Acad. Sci. USA. 104, 11560-11565, 2007. PMID:17878306


Tsugane, M., Nagai, Y., Kimura, Y., Oka, J. and Kimura, H. Differentiated astrocytes acquire sensitivity to hydrogen sulfide that is diminished by the transformation into reactive astrocytes.  Antioxidants & Red. Signal. 9, 257-269, 2007. PMID:17115938

Umemura, K. and Kimura, H.  Hydrogen sulfide enhances reducing activity in neurons: Neurotrophic role of H2S in the brain?  Antioxid. Redox Signal. 9, 2035-2042, 2007. PMID:17822366


Saito, Y., Katori, N., Soyama, A., Nakajima, Y., Yoshitani, T., Kim, S-R., Fukashima-Uesaka, H., Kurose, K., Kaniwa, N., Ozawa, S., Kamatani, N., Komamura, K., Kamakura, S., Kitakaze, M., Tomoike, H., Sugai, K., Minami, N., Kimura, H., Goto, Y. Minami, H., Yshida, T., Kunitoh, H., Ohe, Y., Yamamoto, N., Tamura, T., Saijo, N and Sawada, J.  CYP2C8 haplotype structures and their influence onpharmacokinetics of paclitaxel in a Japanese population.  Pharmacogenetics & Genomics 17, 461-471, 2007. PMID:17558302


Hohjoh H, Fukushima T: Marked change in microRNA expression during neuronal     differentiation of human teratocarcinoma NTera2D1 and mouse embryonal carcinoma P19 cells. BBRC 362: 360-367, 2007


Tamura Y, Kunugi H, Ohashi J, Hohjoh H: Epigenetic aberration of the human REELIN gene in psychiatric disorders. Mol Psychiatry 12: 593-600, 2007


Tamura Y, Kunugi H, Ohashi J, Hohjoh H: The possible association between epigenetic aberration in DNA methylation in RELN and psychiatric disorders. Mol Psychiatry 12: 519, 2007


Hohjoh H, Fukushima T: Expression profile analysis of microRNA (miRNA) in mouse central nervous system using a new miRNA detection system that examines hybridization signals at every step of washing. Gene 391: 39-44, 2007



Umemura, K., Yamashita, N., Yu, X., Arima, K., Asada, T., Makifuchi, T., Murayama, S., Saito, Y., Kanamaru, K., Goto, Y., Kohsaka, S., Kanazawa, I., Kimura, H. Autotaxin expression is enhanced in frontal cortex of Alzheimer-type dementia patients. Neurosci. Lett. 400, 97-100, 2006.


Kimura, Y. Dargusch, R. Schubert, D. and Kimura, H. Hydrogen Sulfide Protects HT22 Neuronal Cells from Oxidative Stress. Antiox. & Red. Signal. 8, 661-670, 2006.


Kaneko, Y., Kimura, Y., Kimura, H. and Niki, I.  L-cysteine inhibits insulin release from the pancreatic b-cell.  Possible involvement of metabolic production of hydrogen sulfide, a novel gasotransmitter.  Diabetes 55, 1391-1397, 2006.


Schicho, R., Krueger, D., Zeller, F., Weyhern, C.W.H.V, Frieling, T., Kimura, H., Ishii, I., Giorgio, R.D., Campi, B. and Schemann, M.  Hydrogen sulfide is a novel prosecretory neuromodulator in the guineapig and human colon.  Gastroenterology 131, 1542-1552, 2006.


Ohashi J., Naka I., Toyoda A., Takasu M., Tokunaga K., Ishida T., Sakaki Y., and Hohjoh H. (2006) Estimation of the species-specific mutation rates of the DRB1 locus in human and chimpanzee. Tissue Antigens, 68: 427-431.


Sakai T. and Hohjoh H. (2006) Gene silencing analyses against amyloid precursor protein (APP) gene family by RNA interference. Cell Biol Int, 30: 952-956.


Kawashima M., Tamiya G., Oka A., Hohjoh H., Juli T., Ebisawa T., Honda Y., Inoko H., and Tokunaga K. (2006) Genome-wide association analysis of human narcolepsy and a new resistance gene. Am J Hum Genet, 79: 252-263.


Ohnishi Y., Tokunaga K., Kaneko K., and Hohjoh H. (2006) Assessment of allele-specific gene silencing by RNA interference with mutant and wild-type reporter alleles. J. RNAi Gene silencing, 2: 154-160




募 集

国立研究開発法人 国立精神・神経医療研究センター 神経研究所


Department of Molecular Pharmacology