Area 1: Main Building, National Institute of Neuroscience
Main Laboratory
- A room where all members' desks and experimental desks are lined up.
Basic lab work is done here.
Laboratory 2
- Room for Western blotting and DNA electrophoresis.
Laboratory 3
- Room with cryostat, FACS and confocal microscope.
Cell Culture Room 1
- Room for cell culture, cell experiment, and muscle tension measurement.
Cell Culture Room 2 (Main Building 5F)
- A room for cell experiments using viruses.
I use it in collaboration with other departments
Cold Room
- 4°C room.
Used in stocks of reagents and in Western and immunostaining reactions.
Discussion Room
- Usually the workplace of secretaries.
We will have a little discussion with each other and have lunch.
Director's Room
- In the director's room, where there are many plants,
researchers exchange ideas actively every day.
Area 2: Major Animal Research Facility 1F
-
- Operation Room
- Laboratory 4
- Laboratory 5
- Cell Culture Room 3
Area 3: Cluster Research Building
Cell Culture Room 4 (iPS Laboratory)
- Experiments using iPS cells and primary cells are performed here.
Laboratory Equipment
Main Laboratory
- Thermal cycler (Applied Biosystems™ Veriti™)
- MultiNA (Shimadzu)
- Fluorescence microscope (KEYENCE BZ-X800)
- Nano Drop (NanoDrop™ One/OneC Ultra Trace UV-Vis Spectrophotometer)
- DNA oven
- Real-time PCR (Applied Biosystems™ StepOnePlus™)
- Zetasizer Nano ZSP (Malvern)
- Spectrophotometer
- Synergy HTX Multimode Plate Reader (BioTek)
- High-performance vibrating blade microtome device
- MilliQ manufacturing equipment
- Draft chamber
Laboratory 2
- Electrophoresis device (Mupid-exU)
- Western blot electrophoresis tank
- Semi-dry blotting device
- Gel imager (NuGenius)
- ChemiDoc™ Imaging System (Bio-Rad)
Laboratory 3
- Cell sorter (SONY SH800S)
- Cryostat
- Confocal microscope
Cell Culture Room 1
- Clean bench
- CO2 incubator
- microscope
- Invitrogen™ Neon™ Transfection System
- Countess Automatic cell counter
Cell Culture Room 2 (Main Building 5F)
- Safety cabinet
- CO2 incubator
- microscope
- Autoclave
Area 2: Major Animal Research Facility 1F
Operation Room
Laboratory 4
- Paraffin embedded block manufacturing equipment
- Paraffin block cooling system
- Sliding microtome
- Specimen block humidifier
- Cryostat
Laboratory 5
- IVIS Imaging System
- Grip strength measuring device
- Treadmill
Cell Culture Room3
Area 3: Cluster Research Building
Cell Culture Room 4 (iPS Laboratory)
- CO2 incubators (Thermo Fisher 370)
- Clean bench
- FACS Aria Fusion (BD)
- CFX Connect Real-time System (BIO-RAD)
- Olympus epifluorescence microscope IX83
- Four inverted routine microscopes (one Leica, one Olympus)
- Ultra low temperature freezer (KM-DU73Y1J, Panasonic), low temperature freezer (MDF-U539, SANYO), 4°C showcase (MPR-1014R, Panasonic)
- XFe24 Extrabeccular Flux analyzer (Seahorse Bioscience) (Cell metabolism kinetic measuring device)
- Real-time sequence analyzer PyroMark Q24 (QIAGEN)
- Autoclave SX-500 (Tommy Seiko)
- Micro high speed cooling centrifuge MX-307 (KUBOTA) (TOMY)
- Centrifuge 4000 (KUBOTA)
- Liquid nitrogen cryopreservation container Taylor-Wharton, LS6000
- Countess Automatic cell counter C100227 (Invitrogen, life technologies)
- Neon gene transfer device MPK5000 (Invitrogen)
